Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity
Figure 4
Specific immune responses to HIV PR75–84 peptide and variants harbouring drug-resistance mutations. Splenocytes were purified two weeks following the last immunization. The cells were stimulated over night with the (a) singly mutated (I84V), (b) doubly mutated (V82F/I84V), or (c) the wild-type peptide variants of PR75–84. Bars are showing the median response of the different groups. Specific responses were calculated by subtracting background responses to a control peptide from PR-specific immune responses. Proportion of responding animals (>50 spot forming cells/106 splenocytes) are indicated above each individual bar.
