International Scholarly Research Notices

Research Article

Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity

Table 1

Summary of rCTB-proteaseepitope fusion protein immunizations.
QuestionImmunized withAmino acid sequence of linked epitopeConclusion
Can the immunogenicity of an epitope be enhanced by linking it to recombinant Cholera toxin 𝛽 -subunit (rCTB)?rCTB-P R 7 5 − 8 9 I84VrCTB-VLVGPTPVNVIGRNLLinking the HIV derived epitope, P R 7 5 − 8 9 / 9 4 , to rCTB efficiently enhanced the immune response to the epitope.
rCTB-P R 7 5 − 9 4 I84VrCTB-VLVGPTPVNVIGRNLLTQIG
rCTB + P R 7 5 − 8 4 V82F/I84VrCTB + VLVGPTPFNV
P R 7 5 − 8 4 V82F/I84VVLVGPTPFNV
rCTBrCTB
Untreated—
Do we need the pentameric form of the chimeric protein for enhancement of immune response?rCTB-P R 7 5 − 8 9 I84VrCTB-VLVGPTPVNVIGRNLPentamerization, permitting GM-1 binding, of the fusion protein correlated with enhanced immune responses to the attached HIV epitope.
rCTB-P R 7 5 − 9 4 I84VrCTB-VLVGPTPVNVIGRNLLTQIG
rCTB-P R 7 5 − 8 4 I84V (monomeric form)rCTB-VLVGPTPVNV
rCTB + P R 7 5 − 8 4 I84VrCTB + VLVGPTPVNV
P R 7 5 − 8 4 I84VVLVGPTPVNV
Untreated—
Can we broaden the immune response by using a variant of the P R 7 5 − 8 4 containing two mutated amino acid residues?rCTB-P R 7 5 − 9 4 V82F/I84VrCTB-VLVGPTPFNVIGRNLLTQIGAddition of the extra mutation reduced the binding property of the fusion protein to GM-1, and resulted in a reduced epitope-specific immune response.
rCTB + P R 7 5 − 8 4 V82F/I84VrCTB + VLVGPTPFNV
P R 7 5 − 8 4 V82F/I84VVLVGPTPFNV
rCTBrCTB
Untreated—