Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity

Table 2

Receptor binding property of the CTB^a moiety of fusion proteins relative to the binding of unmodified CTB.

Group	Fusion protein	Maximal binding	Actual binding	Percent binding^b (%)
A	rCTB-PR75−94I84V	0.79 mg/mL	0.79 mg/mL	100
B	rCTB-PR75−94V82F/I84V	0.86 mg/mL	0.64 mg/mL	74
C	rCTB-PR75−89I84V	0.81 mg/mL	0.75 mg/mL	93
D	rCTB-PR75−84I84V	0.80 mg/mL	0 mg/mL	0

^aCTB: Cholera toxin B subunit.
^b(bound fusion protein/maximal bound rCTB protein) * 100%.