Table of Contents
ISRN Gastroenterology
Volume 2012 (2012), Article ID 176728, 7 pages
http://dx.doi.org/10.5402/2012/176728
Research Article

Lipid Droplet Binding of Hepatitis C Virus Core Protein Genotype 3

1Division of Infectious Diseases, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA
2Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA

Received 15 March 2012; Accepted 27 May 2012

Academic Editors: A. Nakajima and W. Vogel

Copyright © 2012 Guan Qiang and Ravi Jhaveri. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Hepatitis C virus (HCV) genotype 3 is known to cause steatosis (fatty liver) that is more frequent and severe than other genotypes. We previously identified sequence elements within genotype 3 HCV Core domain 3 that were sufficient for lipid accumulation. Aims. We examined various genotype 3 Core domains for lipid droplet localization and compared the lipid droplet binding regions of domain 2 with a genotype 1 isolate. Methods. We generated HCV Core domain constructs fused with green fluorescent protein and performed immunofluorescence to visualize lipid droplets. Results. Constructs containing HCV Core domain 2 are appropriately localized to lipid droplets with varying degrees of efficiency. When compared to genotype 1, there are polymorphisms within domain 2 that do not appear to alter lipid droplet localization. Conclusions. In summary, the differences in a steatosis-associated HCV Core genotype 3 isolate do not appear to involve altered lipid droplet localization.