International Scholarly Research Notices

Research Article

Comparison of LC-MS Assay and HPLC Assay of Busulfan in Clinical Pharmacokinetics Studies

Table 1

Precision and accuracy of the HPLC-FL and LC-MS assay (data is presented as mean ± S.D.).
Busulfan conc. (ng/mL)Intraday ( 𝑛 = 4 )Inter-day ( 𝑛 = 4 )
MeasuredRSD (%)Bias (%)MeasuredRSD (%)Bias (%)
HPLC-FL
31.25 3 2 . 6 ± 3 . 9 12.04.3 3 1 . 8 ± 2 . 2 7.01.8
125.0 1 2 8 . 8 ± 1 0 . 1 7.93.1 1 2 8 . 1 ± 6 . 3 4.92.5
1000.0 1 0 0 0 . 5 ± 3 2 . 0 3.20.05 1 0 1 4 . 2 ± 1 8 . 2 1.71.4
LC-MS
15.6 1 5 . 6 ± 1 . 4 9.20.1 1 6 . 1 ± 0 . 4 2.63.0
125.0 1 2 2 . 4 ± 5 . 3 4.4−2.1 1 2 4 . 6 ± 3 . 3 2.7−0.3
1000.0 1 0 1 1 . 0 ± 1 3 . 6 1.31.1 1 0 1 0 . 6 ± 9 . 6 1.01.1