Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo
Cotransduction and selective expansion of K562 cells at a constant MOI. K562 cells were cotransduced with varying MGMT : GFP virus ratios with the total MOI held at 0.5. (a) Total AGT, total GFP, and dual-positive percentages in the absence of selection. (b) Treatment with 10 μM BG and 15 μM BCNU enriched both AGT only and AGT-GFP dual-positive populations. Total AGT and total GFP values represent the net single and dual-expression percentages for each gene. The expected level of dual-positive cells is based on the product of the total AGT and total GFP expression percentages for each data point. (c) AGT and GFP expression levels measured separately. Transduction with GFP is unaffected by increased MOIs of the MGMT vector. Error bars indicate ±SEM, .
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