Cotransduction with MGMT and Ubiquitous or Erythroid-Specific GFP Lentiviruses Allows Enrichment of Dual-Positive Hematopoietic Progenitor Cells In Vivo
Cotransduction and MGMT-mediated enrichment with limiting MOIs. Whole bone marrow cells from 5-FU-conditioned mice were cotransduced with an MOI of 3 for both MGMT and RRL-GATA-GFP viruses prior to transplant into lethally irradiated recipients. PB AGT+ and TER119+-GFP+ expression percentages were taken at three week intervals, two days prior to each treatment with 30 mg/kg BG, and 10 mg/kg BCNU, as well as in BMMNCs at sacrifice.
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