Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4/NF-𝜿B Signaling Pathway in THP-1 Macrophages

Figure 2

Palmitic acid augmented the level of TLR4 protein and NF-𝜅B p65 phosphorylationprotein in THP-1 macrophages. (a) control; (b) palmitic acid 100 μmol/L group; (c) palmitic acid 200 μmol/L group; (d) palmitic acid 500 μmol/L; (e) LPS 10 ng/mL group. (A) Western blotting electropherogram of TLR4 (96 KD), NF-𝜅B p65 (65 KD), and β-actin (42 KD). (B) Palmitic acid augmented the level of TLR4 protein in a dose-dependent in THP-1 macrophages, with values expressed as mean ± SEM (*𝑃<0.05 versus control). (C) Palmitic acid augmented the level of NF-𝜅B p65 phosphorylation protein in a dose-dependent in THP-1 macrophages, with values expressed as mean ± SEM (^∗𝑃<0.05 versus control).