Table of Contents
ISRN Analytical Chemistry
Volume 2012 (2012), Article ID 245429, 7 pages
http://dx.doi.org/10.5402/2012/245429
Research Article

Simultaneous Estimation of Metoprolol Succinate and Olmesartan Medoxomil in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric Method

Department of Pharmaceutical Chemistry and Analysis, Indukaka Ipcowala College of Pharmacy, Gujarat, New Vallabh Vidyanagar 388121, India

Received 10 March 2012; Accepted 2 May 2012

Academic Editors: L. Nei and M. I. Saleh

Copyright © 2012 Sunil L. Baldania et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method is described for simultaneous analysis of metoprolol succinate and olmesartan medoxomil in pharmaceutical formulations. The separations were achieved on prepared TLC plates precoated with silica gel G 60 F254 and the plates were developed with methanol-ethyl acetate-toluene-glacial acetic acid 2.5 : 3 : 4.5 : 0.3%v/v/vas mobile phase. Detection and evaluation of chromatograms were performed densitometrically at 224 nm. The retention factor of metoprolol succinate and olmesartan medoxomil was 0.25 and 0.70, respectively. The linear range was 1000–2250 ηg per spot for metoprolol succinate and 800–1800 ηg per spot for olmesartan medoxomil and the correlation coefficients (r) were 0.993 and 0.999, respectively. The method was validated for linearity and range, specificity, precision, accuracy, and robustness, and the results were found to be within the acceptance criteria. The method was successfully applied to the analysis of drugs in their capsule dosage form.