International Scholarly Research Notices / 2012 / Article / Tab 3

Research Article

Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form

Table 3

Robustness evaluation of the method ( ).

Factor LevelRetention time ( )Asymmetry (T)
PCMCHZNIMPCMCHZ
NIM

(A) Flow rate (mL min−1)

1.1−12.7004.6259.5821.241.071.12
1.202.7024.6129.5451.181.051.09
1.3+12.6974.5959.4611.151.021.08

Mean ± S.D
2.699 ± 0.04
4.610 ± 0.02
9.529 ± 0.06
1.19 ± 0.04
1.05 ± 0.021.10 ± 0.06

(B) Percentage of acetonitrile in the mobile phase (v/v)

44−12.7104.6509.5671.141.001.11
4502.7024.6129.5451.181.051.09
46+12.6904.5969.5251.101.021.08

Mean ± S.D2.700 ± 0.014.619 ± 0.039.546 ± 0.02
1.13 ± 0.041.03 ± 0.031.09 ± 0.02

(C) Columns from different manufacturers

Hypersil GOLD C182.7024.6129.5451.181.051.09
HiQ-Sil HS C182.6974.620
9.5501.121.18 1.08

Mean ± S.D
2.699 ± 0.03
4.616 ± 0.01
9.547 ± 0.01
1.19 ± 0.03
1.05 ± 0.081.10 ± 0.02