Review Article
Detection and Control of Prion Diseases in Food Animals
Table 2
Comparative analysis of in vitro and ex vivo immunotherapeutic investigations of prion diseases.
| | Region | Model | Efficacy | Reference |
| | mAb 3F4 (109–113) pSera; 23–33, 90–104, 143–156, 219–232 | Cell-free conversion | pSera 219–232 disrupted formation of new PrPSc | Gilch et al. 2003 [178] | | mAb 6H4 (144–152) | ScN2a cell line | Clearance of PrPSc from cell line following antibody treatment | Enari et al. 2001 [177] | | Fab D18 (132–156) | ScN2a cell line | Blocked PrPSc formation and cleared existing PrPSc | Peretz et al. 2001 [179] | In vitro | SAF34 (octarepeat) SAF61 (144–152) | ScN2a cell line overexpressing PrPC | Both Abs decreased PrPSc and PrPC in infected and uninfected cells | Perrier et al. 2004 [180] | | Panel of antibodies to PrPC and PrPSc generated in PrP0/0 mice | ScN2a cell line | Capacity for protection independent of epitope but dependent on ability to bind cell surface PrPC to prevent internalization | Kim et al. 2004 [181] | | ER targeted scFv of 8H4 (175–185) and 8F9 (225–231) | Expression in PC12 cell line | Inhibition of PrPC translocation to cell surface; prevented PrPSc accumulation | Cardinale et al. 2005 [182] | | mAbs to “YYR” motif | ScN2a cell line | Reduced content of PrPSc | Cashman and Caughey 2004 [168] |
| Ex vivo | PrPC poly Ab ex vivo neutralization | Golden hamster | 2-log reduction | Gabizon et al. 1988 [174] | Fab D18 (132–156) Ex vivo neutralization | ScN2a cell line | Mice lived 3 months longer (3-log reduction of infectivity) | Peretz et al. 2001 [179] |
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