Characterization of LlaKI, a New Metal Ion-Independent Restriction Endonuclease from Lactococcus lactis KLDS4

Figure 4

DNA cleavage in the absence of divalent metal ions. Substrate: a 520 bp DNA fragment containing a single recognition site for LlaKI. The restriction enzyme LlaKI cleaved the substrate to yield two DNA fragments of 220 bp and 300 bp. The cleavage reactions were incubated at 30°C for 1 hour.