Research Article
Development and Evaluation of Environmentally Benign Aqueous Two Phase Systems for the Recovery of Proteins from Tannery Waste Water
Table 3
Tie-line data for PEG 10000 (
) + citrate salt (
) + water system at 30°C.
| | Feed | Top phase | Bottom phase | | | | | (% w/w) | (% w/w) | (% w/w) | (% w/w) | (% w/w) | (% w/w) |
TLL% | |
Yield % |
| | PEG 10000 + sodium citrate + water |
| | 22.00 | 9.00 | 29.75 | 1.98 | 2.01 | 21.22 | 33.76 | 0.346 | 84.01 | | 24.00 | 10.00 | 34.03 | 1.51 | 1.02 | 23.21 | 39.50 | 0.308 | 88.30 | | 26.00 | 11.00 | 38.21 | 0.99 | 0.92 | 25.15 | 44.43 | 0.274 | 90.35 | | 28.00 | 12.00 | 40.11 | 0.52 | 0.71 | 28.57 | 48.30 | 0.235 | 95.08 | | 30.00 | 13.00 | 42.02 | 0.23 | 0.51 | 32.33 | 52.47 | 0.216 | 95.86 |
| | PEG 10000 + potassium citrate + water |
| | 22.00 | 9.00 | 32.01 | 2.97 | 1.65 | 19.88 | 34.79 | 0.313 | 80.99 | | 24.00 | 10.00 | 37.22 | 2.54 | 1.12 | 24.11 | 42.12 | 0.265 | 82.03 | | 26.00 | 11.00 | 38.34 | 2.12 | 0.76 | 26.02 | 44.54 | 0.228 | 87.28 | | 28.00 | 12.00 | 40.04 | 1.52 | 0.54 | 27.96 | 47.53 | 0.218 | 91.07 | | 30.00 | 13.00 | 39.98 | 0.98 | 0.43 | 31.03 | 50.46 | 0.188 | 91.98 |
| | PEG 10000 + ammonium citrate + water |
| | 22.00 | 9.00 | 33.23 | 4.23 | 1.23 | 18.89 | 35.24 | 0.211 | 80.96 | | 24.00 | 10.00 | 38.13 | 4.01 | 1.01 | 21.12 | 40.84 | 0.176 | 82.56 | | 26.00 | 11.00 | 41.01 | 3.34 | 0.75 | 23.09 | 44.79 | 0.151 | 85.29 | | 28.00 | 12.00 | 42.96 | 3.03 | 0.51 | 25.11 | 47.85 | 0.126 | 89.61 | | 30.00 | 13.00 | 45.05 | 2.71 | 0.43 | 26.87 | 50.76 | 0.101 | 92.17 |
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