Anticonvulsant Drugs, Brain Glutamate Dehydrogenase Activity and Oxygen Consumption
Figure 1
Effect in vitro of some anticonvulsant drugs on glutamate oxidative deamination catalized by mouse brain glutamate dehydrogenase. Glutamate dehydrogenase activity (GDH) was determined in mouse brain homogenates (1 : 5 w : v on phosphates 0.05 M pH = 7.6 buffer) with different concentrations of pyridoxal phosphate (PPAL), hydroxylamine (OHAMINE) and aminooxyacetic acid (AAOA) with glutamic acid as substrate, using a spectrophotometric method (see Section 2). Results are means ± SEM (). ANOVA test was applied and different characters were used to indicate statistically different groups as compared with the control (, Tuckey’s test).
