Anticonvulsant Drugs, Brain Glutamate Dehydrogenase Activity and Oxygen Consumption
Figure 3
Effect in vitro of aminooxiacetic and hydroxylamine on glutamate dehydrogenase activity of different sources. Effect of different concentrations of aminooxyacetic acid (AAOA), and hydroxylamine (OHAMINE) on reductive amination of α-ketoglutarate and oxidative deamination of glutamate respectively, catalyzed by glutamate dehydrogenase commercially available (from bovine liver), mouse brain, and liver homogenates (1 : 5 w : v on phosphates 0.05 M pH = 7.6 buffer), using a spectrophotometric method (see Section 2). Results are means ± SEM (). ANOVA test was applied, and different characters were used to indicate statistically different groups as compared with the control (, Tuckey’s test).
