International Scholarly Research Notices / 2012 / Article / Fig 3

Research Article

Anticonvulsant Drugs, Brain Glutamate Dehydrogenase Activity and Oxygen Consumption

Figure 3

Effect in vitro of aminooxiacetic and hydroxylamine on glutamate dehydrogenase activity of different sources. Effect of different concentrations of aminooxyacetic acid (AAOA), and hydroxylamine (OHAMINE) on reductive amination of α-ketoglutarate and oxidative deamination of glutamate respectively, catalyzed by glutamate dehydrogenase commercially available (from bovine liver), mouse brain, and liver homogenates (1 : 5 w : v on phosphates 0.05 M pH = 7.6 buffer), using a spectrophotometric method (see Section 2). Results are means ± SEM ( 𝑛 > 4 ). ANOVA test was applied, and different characters were used to indicate statistically different groups as compared with the control ( 𝑃 > 0 . 0 1 , Tuckey’s test).
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