International Scholarly Research Notices / 2012 / Article / Fig 4

Research Article

Anticonvulsant Drugs, Brain Glutamate Dehydrogenase Activity and Oxygen Consumption

Figure 4

Effect of ammonium sulphate on the inhibitory activity of aminooxyacetic acid and hydroxylamine on mouse brain glutamate dehydrogenase. Glutamate dehydrogenase activity (GDH) was determined in mouse brain homogenates (1 : 5 w : v on phosphates 0.05 M pH = 7.6 buffer) for reductive amination of α-ketoglutarate employing different concentrations of ammonium sulphate, with and without aminooxyacetic acid (AAOA, (a)) and hydroxylamine (OHAMINE, (b)), using a spectrophotometric method (see Section 2). Reciprocal of GDH activity are plotted against reciprocal of ammonium concentration.

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