Anticonvulsant Drugs, Brain Glutamate Dehydrogenase Activity and Oxygen Consumption
Figure 4
Effect of ammonium sulphate on the inhibitory activity of aminooxyacetic acid and hydroxylamine on mouse brain glutamate dehydrogenase. Glutamate dehydrogenase activity (GDH) was determined in mouse brain homogenates (1 : 5 w : v on phosphates 0.05 M pH = 7.6 buffer) for reductive amination of α-ketoglutarate employing different concentrations of ammonium sulphate, with and without aminooxyacetic acid (AAOA, (a)) and hydroxylamine (OHAMINE, (b)), using a spectrophotometric method (see Section 2). Reciprocal of GDH activity are plotted against reciprocal of ammonium concentration.
