Inner Membrane Protein YhcB Interacts with RodZ Involved in Cell Shape Maintenance in Escherichia coli
Figure 1
Growth of ΔyhcB ΔrodZ mutants carrying an inducible yhcB gene. (a) Cells of indicated strains grown overnight in LB medium containing 0.1% arabinose were inoculated to give an OD600 of 0.025 into LB medium containing either 0.1% arabinose or 0.2% glucose. Ampicillin was also added at 100 μg/mL to the culture of strain KR0411. Aliquots of culture were taken and OD600 were measured at indicated time intervals. KR0412 (ΔyhcB λ(araC, -yhcB, bla):diamond (black); KR0422 (ΔrodZ λ(araC, -yhcB, bla):square (gray); KR0413 (ΔyhcB ΔrodZ::kanλ(araC, -yhcB, bla):circle (red); KR0423 (ΔrodZ ΔyhcB::kan λ(araC, -yhcB, bla):triangle (orange); KR0411 (ΔyhcB ΔrodZ::kan/pBADs-yhcB):cross (green). Solid (-) and dotted () lines indicate the culture with arabinose and glucose, respectively. (b) Strains KR0412 (ΔyhcB λ(araC, -yhcB, bla)), KR0422 (ΔrodZ λ(araC, -yhcB, bla)), KR0413 (ΔyhcB ΔrodZ::kanλ(araC, -yhcB, bla)), KR0423 (ΔrodZ ΔyhcB::kan λ(araC, -yhcB, bla)), and KR0411 (ΔyhcB ΔrodZ::kan/pBADs-yhcB) were cultured in LB medium containing 0.1% arabinose. Tenfold serial dilutions were made and 5 μL of each were spotted on LB agar plate containing either 0.1% arabinose or 0.2% glucose as indicated, and plates were incubated overnight at 37°C. (c) Cell shape of KR0423 was observed by electron microscopy before (C1) and after (C2) the growth was retarded by YhcB depletion. Horizontal bar is 2 μm.
