Table of Contents
ISRN Analytical Chemistry
Volume 2012 (2012), Article ID 308062, 5 pages
http://dx.doi.org/10.5402/2012/308062
Research Article

Evaluation of the Electrostatic Contribution to the Retention of Modified Nucleosides and Nucleobases by Zwitterionic Hydrophilic Interaction Chromatography

Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Salamanca, 37008 Salamanca, Spain

Received 20 December 2011; Accepted 10 January 2012

Academic Editors: T. Bolanca, A. M. Haji Shabani, and K. Ohyama

Copyright © 2012 Diego García-Gómez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This work explores the benefits and limitations, on a quantitative basis, of using zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) for the separation of several modified nucleosides and nucleobases of clinical interest. The target compounds were hydroxylated and methylated derivatives: 8-hydroxy-guanine, 8-hydroxy-guanosine, 8-hydroxy-2-deoxyguanosine, 1-methyl-guanine, 7-methyl-guanine, and 9-methyl-guanine. A quantitative evaluation of the electrostatic interaction based on a systematic study of the nature and concentration of the salts in the mobile phase has been carried out. From the obtained results, it may be concluded that separation is based on a mechanism of partition and interaction through weak electrostatic forces: the contribution of the electrostatic interaction to the retention of the charged analytes reaching values between 25 and 52% at low salt concentration. However, the electrostatic contribution decreased progressively as the salt concentration rose.