Table of Contents
ISRN Obstetrics and Gynecology
Volume 2012, Article ID 342075, 7 pages
http://dx.doi.org/10.5402/2012/342075
Clinical Study

Evaluation of Cytokines in Endocervical Secretion and Vaginal pH from Women with Bacterial Vaginosis or Human Papillomavirus

1Department of Microbiology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, 74001-970 Goiâna, GO, Brazil
2Discipline of Gynecology and Obstetrics, Oncological Research Institute (IPON), Federal University of Triângulo Mineiro, 38025-440 Uberaba, MG, Brazil
3Discipline of Immunology, Oncological Research Institute (IPON), Federal University of Triângulo Mineiro, 38025-440 Uberaba, MG, Brazil

Received 16 December 2011; Accepted 7 January 2012

Academic Editors: R. M. Austin and C. Romero

Copyright © 2012 Ana Claudia Camargo Campos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To verify the relationship between vaginal pH and human papillomavirus (HPV) infection and to measure cytokine levels in endocervical secretions of women with bacterial vaginosis (BV) or HPV. Methods. 173 women (16–48 years old) were enrolled and divided into groups: BV, HPV, and controls. Microbiological culture and vaginal pH were measured. HPV detect by PCR, and cytokines by ELISA (IL-2, IL-6, IL-10, IL-12, TNF-α, and IFN-γ cytokines). Results. Of 173 women, 60 were control group (34.7%) and 113 were distributed in HPV ( 𝑛 = 3 6 , 20.8%), BV ( 𝑛 = 3 6 , 20.8%), vaginitis ( 𝑛 = 3 0 , 17.3%) and, BV and HPV-associated groups ( 𝑛 = 1 1 , 6.4%). Vaginal pH > 4.5 was related with HPV infection. IL-2 and IL-12 were increased in BV and HPV groups, and IL-6 (only BV group), compared to control group. IL-12 and IFN-γ were higher in HPV than BV group. Conclusion. The increase of vaginal pH was associated with HPV infection; BV and HPV groups had a Th1 cytokines immune response.