Modulation of Neutrophil Apoptosis by Antimicrobial Peptides

Figure 10

Effect of a P2Y₆ antagonist on the HNP-1-induced suppression of neutrophil apoptosis. Neutrophils (10⁶ cells/mL) were incubated for 18 h at 37°C in RPMI1640-10%FBS in the absence (Control) or presence of HNP-1, -2, and -3 (20 μg/mL each), UDP (300 μM), MRS2578 (1 μM), or their combination (+; 20 μg/mL HNP-1, -2, or -3 and 1 μM MRS2578, or 300 μM UDP, and 1 μM MRS2578). Neutrophils were also incubated alone for 18 h at 4°C in the absence of HNPs, UDP or MRS2578 (Resting). After incubation, apoptosis of neutrophils was quantitated and expressed as a percentage of apoptotic cells. Data are the mean ± SD of 3 to 5 separate experiments. Values are compared between the incubation at 37°C with HNPs or UDP in the absence (−) and presence (+) of MRS2578 (d). **𝑃<0.01; ***𝑃<0.001.