Table of Contents
ISRN Hematology
Volume 2012 (2012), Article ID 358316, 6 pages
http://dx.doi.org/10.5402/2012/358316
Research Article

RhC Phenotyping, Adsorption/Elution Test, and SSP-PCR: The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors

1Graduate Program in Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Campus, Rangsit, Patumthani 12121, Thailand
2Division of Blood Bank, Department of Pathology, Phramongkutklao Hospital, Bangkok 10400, Thailand
3Division of Immunology, Department of Clinical Pathology, Army Institute of Pathology, Bangkok 10400, Thailand
4Department of Biochemistry, Phramongkutklao College of Medicine, Bangkok 10400, Thailand
5Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Campus, Rangsit, Patumthani 12121, Thailand

Received 22 September 2012; Accepted 14 October 2012

Academic Editors: A. Pekrun and A. M. Will

Copyright © 2012 Songsak Srijinda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. F. F. Wagner and W. A. Flegel, “RHCE represents the ancestral RH position, while RHD is the duplicated gene,” Blood, vol. 99, no. 6, pp. 2272–2273, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. I. Mouro, Y. Colin, B. C. Z. J. P. Cartron, and C. Le Van Kim, “Molecular genetic basis of the human Rhesus blood group system,” Nature Genetics, vol. 5, no. 1, pp. 62–65, 1993. View at Publisher · View at Google Scholar · View at Scopus
  3. H. Okuda, H. Suganuma, T. Kamesaki et al., “The analysis of nucleotide substitutions, gaps, and recombination events between RHD and RHCE genes through complete sequencing,” Biochemical and Biophysical Research Communications, vol. 274, no. 3, pp. 670–683, 2000. View at Publisher · View at Google Scholar · View at Scopus
  4. F. F. Wagner and W. A. Flegel, “RHD gene deletion occurred in the Rhesus box,” Blood, vol. 95, no. 12, pp. 3662–3668, 2000. View at Google Scholar · View at Scopus
  5. F. F. Wagner, A. Frohmajer, and W. A. Flegel, “RHD positive haplotypes in D negative Europeans,” BMC Genetics, vol. 2, article 10, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. B. K. Singleton, C. A. Green, N. D. Avent et al., “The presence of an RHD pseudogene containing a 37 base pair duplication and a nonsense mutation in africans with the Rh D-negative blood group phenotype,” Blood, vol. 95, no. 1, pp. 12–18, 2000. View at Google Scholar · View at Scopus
  7. J. C. Chen, T. M. Lin, Y. L. Chen, Y. H. Wang, Y. T. Jin, and C. T. Yue, “RHD 1227A is an important genetic marker for RhDel individuals,” The American Journal of Clinical Pathology, vol. 122, no. 2, pp. 193–198, 2004. View at Publisher · View at Google Scholar · View at Scopus
  8. H. Okuda, M. Kawano, S. Iwamoto et al., “The RHD gene is highly detectable in RhD-negative Japanese donors,” The Journal of Clinical Investigation, vol. 100, no. 2, pp. 373–379, 1997. View at Google Scholar · View at Scopus
  9. P. Perco, C. P. Shao, W. R. Mayr, S. Panzer, and T. J. Legler, “Testing for the D zygosity with three different methods revealed altered Rhesus boxes and a new weak D type,” Transfusion, vol. 43, no. 3, pp. 335–339, 2003. View at Publisher · View at Google Scholar · View at Scopus
  10. C. F. Sun, C. S. Chou, N. C. Lai, and W. T. Wang, “RHD gene polymorphisms among RhD-Negative Chinese in Taiwan,” Vox Sanguinis, vol. 75, no. 1, pp. 52–57, 1998. View at Publisher · View at Google Scholar · View at Scopus
  11. Y. Okubo, H. Yamaguchi, T. Tomita, and N. Nagao, “A D variant, Del?” Transfusion, vol. 24, no. 6, p. 542, 1984. View at Google Scholar · View at Scopus
  12. K. H. Mak, K. F. Yan, S. S. Cheng, and M. Y. Yuen, “Rh phenotypes of Chinese blood donors in Hong Kong, with special reference to weak D antigens,” Transfusion, vol. 33, no. 4, pp. 348–351, 1993. View at Google Scholar · View at Scopus
  13. C. P. Shao, J. H. Maas, Y. Q. Su, M. Köhler, and T. J. Legler, “Molecular background of Rh D-positive, D-negative, Del and weak D phenotypes in Chinese,” Vox Sanguinis, vol. 83, no. 2, pp. 156–161, 2002. View at Publisher · View at Google Scholar · View at Scopus
  14. J. C. Lan, Q. Chen, D. L. Wu, H. Ding, D. B. Pong, and T. Zhao, “Genetic polymorphism of RhD-negative associated haplotypes in the Chinese,” Journal of Human Genetics, vol. 45, no. 4, pp. 224–227, 2000. View at Google Scholar · View at Scopus
  15. Q. Li, L. Y. Ye, Z. H. Guo, M. Qian, and Z. Y. Zhu, “Study on the molecular background of Del phenotype in Chinese population,” Chinese Journal of Medical Genetics, vol. 23, no. 5, pp. 486–491, 2006 (Chinese). View at Google Scholar · View at Scopus
  16. Y. Fukumori, Y. Hori, S. Ohnoki et al., “Further analysis of D(e1) (D-elute) using polymerase chain reaction (PCR) with RHD gene-specific primers,” Transfusion Medicine, vol. 7, no. 3, pp. 227–231, 1997. View at Google Scholar · View at Scopus
  17. Y. H. Wang, J. C. Chen, K. T. Lin, Y. J. Lee, Y. F. Yang, and T. M. Lin, “Detection of RhDel in RhD-negative persons in clinical laboratory,” Journal of Laboratory and Clinical Medicine, vol. 146, no. 6, pp. 321–325, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. Y. K. Ji, Y. K. Sung, C. A. Kim, S. Y. Gyu, and S. P. Sung, “Molecular characterization of D- Korean persons: development of a diagnostic strategy,” Transfusion, vol. 45, no. 3, pp. 345–352, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. T. Wagner, G. F. Körmö, C. Buchta et al., “Anti-D immunization by DEL red blood cells,” Transfusion, vol. 45, no. 4, pp. 520–526, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. H. Yasuda, H. Ohto, S. Sakuma, and Y. Ishikawa, “Secondary anti-D immunization by Del red blood cells,” Transfusion, vol. 45, no. 10, pp. 1581–1584, 2005. View at Google Scholar · View at Scopus
  21. K. H. Kim, K. E. Kim, K. S. Woo, J. Y. Han, J. M. Kim, and K. U. Park, “Primary anti-D immunization by DEL red blood cells,” Korean Journal of Laboratory Medicine, vol. 29, no. 4, pp. 361–365, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. E. Rochna and N. C. Hughes-Jones, “The use of purified 125-I-labelled anti-gamma globulin in the determination of the number of D antigen sites on red cells of different phenotypes,” Vox Sanguinis, vol. 10, no. 6, pp. 675–686, 1965. View at Google Scholar · View at Scopus