Efficient Nonviral Gene Therapy Using Folate-Targeted Chitosan-DNA Nanoparticles In Vitro
Figure 6
Intracellular trafficking in KB cells. KB cells were incubated with chitosan nanoparticle complex for 2 hours 37°C, fixes and examined by confocal microscopy. A1 and B1: endosomes and lysosomes immunolabeled with antiearly endosome maker (EEA-1) and lysosomal-associated membrane protein1 (LAMP-1) and fluorescent-labeled anti-mouse antibodies appear green. A2 and B2: plasmid DNA binded with Propidium Iodide (PI) appears red. Stained DNA were purified before nanoparticle; synthesis. A3 and B3: bleu located nuclei of cells as DAPI staining. Under the same condition of transfection with chitosan nanoparticule, A1, B1: endosomes and lysosomes A4 and B4. Superposition of different staining for a three-dimensional image reconstruction. (c) Whole cells, (d) nuclei of KB were isolated. Red shows plasmid DNA beforehand stained with PI; no any staining is there for nuclear DNA.
