Table of Contents
ISRN Pharmaceutics
Volume 2012 (2012), Article ID 374212, 7 pages
http://dx.doi.org/10.5402/2012/374212
Research Article

Accumulation and Elimination of Enrofloxacin and Ciprofloxacin in Tissues of Shrimp Litopenaeus vannamei under Laboratory and Farm Conditions

Research Center for Food and Development AC, Coordination of Food Science, Carretera a la Victoria Km. 0.6, 83000 Hermosillo, SON, Mexico

Received 29 March 2012; Accepted 19 April 2012

Academic Editors: C. Biot and Y. Cheng

Copyright © 2012 Brisa Marisol Flores-Miranda et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to quantify the accumulation and elimination of Enrofloxacin (ENRO) and Ciprofloxacin (CIPRO) in cultivated Litopenaeus vannamei under controlled laboratory and farm conditions. Laboratory- and farm-raised shrimp were given feed supplemented with 200 mg/kg ENRO for 14 days, followed by a 16-day diet without antibiotics. The levels of ENRO and CIPRO were analyzed by High Performance Liquid Chromatography (HPLC). In the laboratory, ENRO concentrations in the muscle and hepatopancreas reached a maximum ( C m a x ) of 0 . 5 4 ± 0 . 2 6 μg/g and 3 . 5 2 ± 1 . 9 μg/g, respectively; C m a x values for CIPRO in the laboratory were 0 . 1 8 ± 0 . 1 3 μg/g (muscle) and 1 . 0 5 ± 0 . 2 0 μg/g (hepatopancreas). In farmed shrimp, C m a x values for ENRO were 0 . 3 6 ± 0 . 1 7 μg/g muscle and 1 . 6 0 ± 0 . 8 2 μg/g in the hepatopancreas; CIPRO C m a x values were 0 . 0 3 ± 0 . 0 2 μg/g (muscle) and 0 . 3 6 ± 0 . 0 8 μg/g (hepatopancreas). Two to fourteen days were necessary to eliminate both antibiotics from muscular tissue and four to more fourteen days for complete elimination of the antibiotics from the hepatopancreas. These results should be considered in terms of minimum concentrations necessary to inhibit Vibrio bacteria to determine whether the current use of this antibiotic is effective in controlling disease.