Table of Contents
ISRN Oncology
Volume 2012, Article ID 382010, 6 pages
http://dx.doi.org/10.5402/2012/382010
Review Article

Epithelial Mesenchymal Transition: A New Insight into the Detection of Circulating Tumor Cells

Clinical Laboratory, Astralab Department of Specialized Analyses, 87000 Limoges, France

Received 15 December 2011; Accepted 13 February 2012

Academic Editors: G. Ghanem, L. Saragoni, M. Stracke, and L.-M. Sun

Copyright © 2012 Guislaine Barrière et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Kalluri and R. A. Weinberg, “The basics of epithelial-mesenchymal transition,” Journal of Clinical Investigation, vol. 119, no. 6, pp. 1420–1428, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. M. W. Klymkowsky and P. Savagner, “Epithelial-mesenchymal transition: a cancer researcher's conceptual friend and foe,” American Journal of Pathology, vol. 174, no. 5, pp. 1588–1593, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. E. Tomaskovic-Crook, E. W. Thompson, and J. P. Thiery, “Epithelial to mesenchymal transition and breast cancer,” Breast Cancer Research, vol. 11, no. 6, article 213, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. D. Sarrió, S. M. Rodriguez-Pinilla, D. Hardisson, A. Cano, G. Moreno-Bueno, and J. Palacios, “Epithelial-mesenchymal transition in breast cancer relates to the basal-like phenotype,” Cancer Research, vol. 68, no. 4, pp. 989–997, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. J. Yang, S. A. Mani, J. L. Donaher et al., “Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis,” Cell, vol. 117, no. 7, pp. 927–939, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. D. Hanahan and R. A. Weinberg, “The hallmarks of cancer,” Cell, vol. 100, no. 1, pp. 57–70, 2000. View at Google Scholar · View at Scopus
  7. A. F. Chambers, A. C. Groom, and I. C. MacDonald, “Dissemination and growth of cancer cells in metastatic sites,” Nature Reviews Cancer, vol. 2, no. 8, pp. 563–572, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. T. R. Ashworth, “A case of cancer in which cells similar to those in the tumors were seen in the blood after death,” Australian Medicine Journal, vol. 14, pp. 146–149, 1869. View at Google Scholar
  9. B. Aktas, M. Tewes, T. Fehm, S. Hauch, R. Kimmig, and S. Kasimir-Bauer, “Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients,” Breast Cancer Research, vol. 11, no. 4, article R46, 2009. View at Publisher · View at Google Scholar · View at Scopus
  10. P. A. Theodoropoulos, H. Polioudaki, S. Agelaki et al., “Circulating tumor cells with a putative stem cell phenotype in peripheral blood of patients with breast cancer,” Cancer Letters, vol. 288, no. 1, pp. 99–106, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. A. J. Armstrong, M. S. Marengo, S. Oltean et al., “Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers,” Molecular Cancer Research, vol. 9, no. 8, pp. 997–1007, 2011. View at Publisher · View at Google Scholar
  12. G. Kallergi, M. A. Papadaki, E. Politaki, D. Mavroudis, V. Georgoulias, and S. Agelaki, “Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patients,” Breast Cancer Research, vol. 13, no. 3, article R59, 2011. View at Publisher · View at Google Scholar
  13. A. Lecharpentier, P. Vielh, P. Perez-Moreno, D. Planchard, J. C. Soria, and F. Farace, “Detection of circulating tumour cells with a hybrid (epithelial/ mesenchymal) phenotype in patients with metastatic non-small cell lung cancer,” British Journal of Cancer, vol. 105, no. 9, pp. 1338–1341, 2011. View at Publisher · View at Google Scholar
  14. M. Mego, S. A. Mani, B.-N. Lee et al., “Expression of epithelial-mesenchymal transition-inducing transcription factors in primary breast cancer: the effect of neoadjuvant therapy,” International Journal of Cancer, vol. 130, no. 4, pp. 808–816, 2012. View at Publisher · View at Google Scholar
  15. C. V. Pecot, F. Z. Bischoff, J. A. Mayer et al., “A novel platform for detection of CK+ and CK- CTCs,” Cancer Discovery, vol. 1, no. 7, pp. 580–586, 2011. View at Google Scholar
  16. G. Barrière, A. Riouallon, J. Renaudie et al., “Mesenchymal and stemness circulating tumor cells in early breast cancer diagnosis,” BMC Cancer, vol. 12, no. 1, p. 114, 2012. View at Google Scholar
  17. J. S. Ross and E. A. Slodkowska, “Circulating and disseminated tumor cells in the management of breast cancer,” American Journal of Clinical Pathology, vol. 132, no. 2, pp. 237–245, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. S. Zhao, Y. Liu, Q. Zhang et al., “The prognostic role of circulating tumor cells (CTCs) detected by RT-PCR in breast cancer: a meta-analysis of published literature,” Breast Cancer Research and Treatment, vol. 130, no. 3, pp. 809–816, 2011. View at Publisher · View at Google Scholar
  19. M. C. Liu, P. G. Shields, R. D. Warren et al., “Circulating tumor cells: a useful predictor of treatment efficacy in metastatic breast cancer,” Journal of Clinical Oncology, vol. 27, no. 31, pp. 5153–5159, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. M. Mego, U. D. Giorgi, S. Dawood et al., “Characterization of metastatic breast cancer patients with nondetectable circulating tumor cells,” International Journal of Cancer, vol. 129, no. 2, pp. 417–423, 2011. View at Publisher · View at Google Scholar
  21. C. E. Denlinger, J. S. Ikonomidis, C. E. Reed, and F. G. Spinale, “Epithelial to mesenchymal transition: the doorway to metastasis in human lung cancers,” Journal of Thoracic and Cardiovascular Surgery, vol. 140, no. 3, pp. 505–513, 2010. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Vazquez-Martin, C. Oliveras-Ferraros, S. Cufí, S. Del Barco, B. Martin-Castilloand, and J. A. Menendez, “Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status,” Cell Cycle, vol. 9, no. 18, pp. 3807–3814, 2010. View at Publisher · View at Google Scholar · View at Scopus
  23. M. S. Wicha and D. F. Hayes, “Circulating tumor cells: not all detected cells are bad and not all bad cells are detected,” Journal of Clinical Oncology, vol. 29, no. 12, pp. 1508–1511, 2011. View at Publisher · View at Google Scholar
  24. S. Tveito, K. Andersen, R. Kåresen, and Ø. Fodstad, “Analysis of EpCAM positive cells isolated from sentinel lymph nodes of breast cancer patients identifies subpopulations of cells with distinct transcription profiles,” Breast Cancer Research, vol. 13, no. 4, article R75, 2011. View at Publisher · View at Google Scholar
  25. A. M. Sieuwerts, J. Kraan, J. Bolt et al., “Anti-epithelial cell adhesion molecule antibodies and the detection of circulating normal-like breast tumor cells,” Journal of the National Cancer Institute, vol. 101, no. 1, pp. 61–66, 2009. View at Publisher · View at Google Scholar · View at Scopus
  26. M. Al-Hajj, M. S. Wicha, A. Benito-Hernandez, S. J. Morrison, and M. F. Clarke, “Prospective identification of tumorigenic breast cancer cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 7, pp. 3983–3988, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. M. J. Meyer, J. M. Fleming, M. A. Ali, M. W. Pesesky, E. Ginsburg, and B. K. Vonderhaar, “Dynamic regulation of CD24 and the invasive, CD44posCD24neg phenotype in breast cancer cell lines,” Breast Cancer Research, vol. 11, no. 6, article R82, 2009. View at Publisher · View at Google Scholar · View at Scopus
  28. E. Charafe-Jauffret, C. Ginestier, F. Iovino et al., “Breast cancer cell lines contain functional cancer stem sells with metastatic capacity and a distinct molecular signature,” Cancer Research, vol. 69, no. 4, pp. 1302–1313, 2009. View at Publisher · View at Google Scholar · View at Scopus
  29. D. S. Micalizzi, S. M. Farabaugh, and H. L. Ford, “Epithelial-mesenchymal transition in cancer: parallels between normal development and tumor progression,” Journal of Mammary Gland Biology and Neoplasia, vol. 15, no. 2, pp. 117–134, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. M. K. Wendt, T. M. Allington, and W. P. Schiemann, “Mechanisms of the epithelial-mesenchymal transition by TGF-β,” Future Oncology, vol. 5, no. 8, pp. 1145–1168, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. F.A. Mamuya and M.K. Duncan, “αV integrins and TGF-β induced EMT; a circle of regulation,” Journal of Cellular and Molecular Medicine, vol. 16, no. 3, pp. 445–455, 2012. View at Google Scholar
  32. C. J. Creighton, J. C. Chang, and J. M. Rosen, “Epithelial-mesenchymal transition (EMT) in tumor-initiating cells and its clinical implications in breast cancer,” Journal of Mammary Gland Biology and Neoplasia, vol. 15, no. 2, pp. 253–260, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. S. Howard, T. Deroo, Y. Fujita, and N. Itasaki, “A positive role of cadherin in wnt/β-catenin signalling during epithelial-mesenchymal transition,” PLoS ONE, vol. 6, no. 8, Article ID e23899, 2011. View at Publisher · View at Google Scholar
  34. N. Takebe, R. Q. Warren, and S. P. Ivy, “Breast cancer growth and metastasis: interplay between cancer stem cells, embryonic signaling pathways and epithelial-to-mesenchymal transition,” Breast Cancer Research, vol. 13, no. 3, p. 211, 2011. View at Google Scholar
  35. M. Katoh, “Network of WNT and other regulatory signaling cascades in pluripotent stem cells and cancer stem cells,” Current Pharmaceutical Biotechnology, vol. 12, no. 2, pp. 160–170, 2011. View at Google Scholar
  36. A. G. De Herreros, S. Peiró, M. Nassour, and P. Savagner, “Snail family regulation and epithelial mesenchymal transitions in breast cancer progression,” Journal of Mammary Gland Biology and Neoplasia, vol. 15, no. 2, pp. 135–147, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. O. Schmalhofer, S. Brabletz, and T. Brabletz, “E-cadherin, β-catenin, and ZEB1 in malignant progression of cancer,” Cancer and Metastasis Reviews, vol. 28, no. 1-2, pp. 151–166, 2009. View at Publisher · View at Google Scholar · View at Scopus
  38. D. A. Cantrell, “Phosphoinositide 3-kinase signalling pathways,” Journal of Cell Science, vol. 114, no. 8, pp. 1439–1445, 2001. View at Google Scholar · View at Scopus
  39. N. M. Chau and M. Ashcroft, “Akt2: a role in breast cancer metastasis,” Breast Cancer Research, vol. 6, no. 1, pp. 55–57, 2004. View at Publisher · View at Google Scholar · View at Scopus
  40. G. Z. Cheng, S. Park, S. Shu et al., “Advances of AKT pathway in human oncogenesis and as a target for anti-cancer drug discovery,” Current Cancer Drug Targets, vol. 8, no. 1, pp. 2–6, 2008. View at Publisher · View at Google Scholar · View at Scopus
  41. R. J. O. Dowling, P. J. Goodwin, and V. Stambolic, “Understanding the benefit of metformin use in cancer treatment,” BMC Medicine, vol. 9, article 33, 2011. View at Publisher · View at Google Scholar
  42. M. H. Yang, D. S. S. Hsu, H. W. Wang et al., “Bmi1 is essential in Twist1-induced epithelial-mesenchymal transition,” Nature Cell Biology, vol. 12, no. 10, pp. 982–992, 2010. View at Publisher · View at Google Scholar · View at Scopus
  43. P. S. Mongroo and A. K. Rustgi, “The role of the miR-200 family in epithelial-mesenchymal transition,” Cancer Biology and Therapy, vol. 10, no. 3, pp. 219–222, 2010. View at Publisher · View at Google Scholar · View at Scopus
  44. M. Xiong, L. Jiang, Y. Zhou et al., “The miR-200 family regulates TGF-β1-induced renal tubular epithelial to mesenchymal transition through smad pathway by targeting ZEB1 and ZEB2 expression,” American Journal of Physiology, vol. 302, no. 3, pp. F369–F379, 2012. View at Publisher · View at Google Scholar
  45. C. Oliveras-Ferraros, S. Cufí, A. Vazquez-Martin et al., “Micro(mi)RNA expression profile of breast cancer epithelial cells treated with the anti-diabetic drug metformin: induction of the tumor suppressor miRNA let-7a and suppression of the TGFβ-induced oncomiR miRNA-181a,” Cell Cycle, vol. 10, no. 7, pp. 1144–1151, 2011. View at Publisher · View at Google Scholar
  46. J. Persson, M. Bäckström, H. Johansson, K. Jirström, G. C. Hansson, and M. Ohlin, “Molecular evolution of specific human antibody against muc1 mucin results in improved recognition of the antigen on tumor cells,” Tumor Biology, vol. 30, no. 4, pp. 221–231, 2009. View at Publisher · View at Google Scholar · View at Scopus
  47. S. S.A. Hamid and S. H. Cheah, “Generation and characterization of a high-affinity monoclonal antibody for MUC1 measurement in breast cancer,” Hybridoma, vol. 30, no. 2, pp. 137–143, 2011. View at Publisher · View at Google Scholar
  48. T. Deguchi, M. Tanemura, E. Miyoshi et al., “Increased immunogenicity of tumor-associated antigen, mucin 1, engineered to express α-Gal epitopes: a novel approach to immunotherapy in pancreatic cancer,” Cancer Research, vol. 70, no. 13, pp. 5259–5269, 2010. View at Publisher · View at Google Scholar · View at Scopus
  49. M. A. Tarp, A. L. Sørensen, U. Mandel et al., “Identification of a novel cancer-specific immunodominant glycopeptide epitope in the MUC1 tandem repeat,” Glycobiology, vol. 17, no. 2, pp. 197–209, 2007. View at Publisher · View at Google Scholar · View at Scopus
  50. G. Medoro, S. Gross, N. Manaresi et al., “Use of the DEPArray platform to detect, isolate, and molecularly characterize pure tumor cells from peripheral blood samples enriched using the CellSearch system,” Journal of Clinical Oncology, vol. 29, supplement, abstrat 10616, 2011. View at Google Scholar
  51. A. Ståhlberg, M. Kubista, and P. Åman, “Single-cell gene-expression profiling and its potential diagnostic applications,” Expert Review of Molecular Diagnostics, vol. 11, no. 7, pp. 735–740, 2011. View at Publisher · View at Google Scholar