Epithelial Mesenchymal Transition: A New Insight into the Detection of Circulating Tumor Cells

Table 1

EMT-derived ddCTC. ddCTC patterns from epithelial differentiation to mesenchymal phenotype are summarized by data reported in quoted publications. Epithelial and mesenchymal markers can be coexpressed.

Authors	Cell enrichment and technology	Analysed characteristics of CTC subpopulations
		Epithelial	Mesenchymal	Stemness
Aktas et al. [9]	AdnaGen and RTPCR; Density gradient centrifugation		Twist1, AKT2, PI3Ka	ALDH1; CD44^+/CD24^−low
Theodoropoulos et al. [10]	Cytospin,; Immunofluorescence microscopy	CK		ALDH1
Armstrong et al. [11]	CellSearch	EpCAM, CK,; E cadherin	Vimentin,; Ncadherin, O cadherin	CD133
Kallergi et al [12]	Density gradient centrifugation; Cytospin and confocal microscopy	CK	Twist1, Vimentin
Lecharpentier et al. [13]	ISET filtration	panCK	Vimentin
Mego et al [14]	Depletion of EpCAM; and CD45+cells RTPCR		Twist, SNAIL1, SLUG; ZEB1, FOXC2
Pecot et al. [15]	Microchannel platform	CK	Vimentin, Twist; Ncadherin, SNAIL
Barrière et al. [16]	AdnaGen and RTPCR		Twist1, AKT2, PI3Ka	ALDH1; BMI1, CD44