Table of Contents
ISRN Pharmaceutics
Volume 2012, Article ID 404606, 6 pages
Clinical Study

Cytological Aspects on the Effects of a Nasal Spray Consisting of Standardized Extract of Citrus Lemon and Essential Oils in Allergic Rhinopathy

1Department of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, Italy
2Department of Food Science, Faculty of Agriculture, University of Naples Federico II, Via Università 100, 80055 Portici (Naples), Italy
3ENT Department, AIAS Structure of Afragola, Contrada Leutrec snc, 80021 Naples, Italy

Received 26 September 2012; Accepted 16 October 2012

Academic Editors: K. Cal, F.-R. Chang, and J. Peng

Copyright © 2012 Lydia Ferrara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this paper, a new formulation of nasal spray was set up based on the extract of lemon pulp, obtained by using a new solid-liquid technology of extraction, added to pure Aloe juice, soluble propoli, and essential oils of Ravensara and Niaouly. It was tested in a clinical study in which 100 subjects were recruited for a period of one month. Nasal scraping was used for collecting samples and after the application of the May-Grünwald Giemsa standard technique, glass slides were analysed by using optical microscope with a 1000x oil immersion. A control group constituted of ten people was recruited as control and this group was administered with physiological solution (saline solution). The comparison of results obtained before and after the application of nasal spray showed a total reduction of eosinophils granulocytes and mast cells; clinical data were confirmed by improvement of clinical pictures of patients. The lemon-based nasal spray was a good alternative to conventional medicine for the treatment of perennial and seasonal allergic and vasomotor rhinopathy.