Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization

Figure 4

LNA and phosphorohioate effects on AMO activity and specificity. Fold increase of luciferase activity after AMO treatment at 100 nM concentrations. The amount of LNA monomers incorporated in the oligonucleotides used varied between 0% and 50%. No significant disparity in activity was seen between 32% and 50% LNA content. The activity of AMOs with 32% and 50% LNA was significantly greater than with 0% LNA. Phosphorothioate AMO with 32% LNA and AMO absent of LNA both showed a significant difference in activity between wildtype and mismatched construct.