Modulating Anti-MicroRNA-21 Activity and Specificity Using Oligonucleotide Derivatives and Length Optimization
Oligonucleotides used in this study.The anti-miR21 ONs used in this study, ranging from 9 to 22 nucleotides in length. The ONs are 2′OMe RNA, LNA/2′OMe RNA mixmers with or without phosphorothioate backbone, and LNA/2′OMe/UNA mixmers. Corresponding ONs with 2MMs were synthesized. Mismatched bases are mismatched against the bases 9 and 11 in the target miR21 sequence. Phosphorothioate AMOs are denoted with PS, ONs lacking this denotation are phosphodiester AMOs. Capital letters represent 2′OMe ribonucleotides. Lowercase letters indicate LNA monomers, whereas underlined letters represent UNA monomers. Italic letters mark mismatches and bold letters phosphorothioates.