Table 1: Encapsulation efficiency of Nos in NT-IO and hATF-Cy5.5-IO NPs.


% Encapsulated drug98.7 ± 0.788.2 ± 2.3
Number of Nos molecules per IO NP*740 ± 98558 ± 74

T otal number of IONPs in each sample was derived from measuring absorption of OD500 of Fe concentration and then calculating the numbers of IONPs using (1). For example, 1 mg of Fe equals to 0.906 nmol of 10 nm IONPs. The numbers of Nos molecules in each IO nanoparticle were calculated by dividing total number of Nos with the total number of IONPs in each sample. Data from Abdalla et al. [30].