Table of Contents
ISRN Analytical Chemistry
Volume 2012 (2012), Article ID 414075, 7 pages
http://dx.doi.org/10.5402/2012/414075
Research Article

Stability-Indicating HPTLC-Densitometric Method for Estimation of Dorzolamide Hydrochloride in Eye Drops

1Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth University, Maharashtra, Pune 411038, India
2Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, , , RAK Medical & Health Sciences University, P.O. Box 11172, Ras Al-Khaimah, UAE

Received 26 March 2012; Accepted 7 May 2012

Academic Editors: J. N. Latosinska and K. Ohyama

Copyright © 2012 S. S. Havele and S. R. Dhaneshwar. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study describes the degradation of dorzolamide HCl under different International Conference on Harmonization prescribed stress conditions (hydrolysis, oxidation, thermolysis, and photolysis) and application of a specific and selective stability-indicating chromatography assay. HPTLC was done on silica Gel 60 F254 TLC plate using chloroform: methanol in the ratio of 9.0 : 1.0 (v/v) as mobile phase and detection at 253 nm.