Stability-Indicating HPTLC-Densitometric Method for Estimation of Dorzolamide Hydrochloride in Eye Drops

Table 8

Summary of stress study.

Parameter	Condition
	Acid 1 M HCl, ref ^a(60°C)	Base 0.2 M NaOH, ref ^a(60°C)	H2O2 6% (w/v)	UV light
Time (hrs)	2	4.5	72	96
Assay of active substance %	88.02	77.96	72.31	84.95
Mass balance	99.99	99.92	99.98	99.96
Degradation (%)	22.25	18.53	22.88	0.80
𝑅𝑓 of major degradation product	0.33	0.32	0.66	0.64

^aRefluxed for 60°C.