Table of Contents
ISRN Veterinary Science
Volume 2012 (2012), Article ID 419378, 6 pages
http://dx.doi.org/10.5402/2012/419378
Research Article

Hemoculture and Polymerase Chain Reaction Using Primers TCZ1/TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis

1Departamento de Doenças Tropicais e Diagnóstico por Imagem, Faculdade de Medicina de Botucatu (FMB), Universidade Estadual Paulista (UNESP), 18618970 Botucatu, SP, Brazil
2Agência Paulista de Tecnologia dos Agronegócios (APTA) Pólo Centro Oeste, 17030-000 Bauru, SP, Brazil

Received 17 February 2012; Accepted 10 April 2012

Academic Editors: A. Anadon and M. H. Kogut

Copyright © 2012 Luciano José Eloy and Simone Baldini Lucheis. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. American trypanosomiasis, also known as Chagas disease, is a zoonosis caused by Trypanosoma cruzi (T. cruzi). Dogs and cats participate actively in this parasite's transmission cycle. This study aimed at evaluating the occurrence of T. cruzi in dogs and cats from Botucatu, SP, Brazil, as well as at evaluating the technique of hemoculture in LIT (liver infusion tryptose) medium by polymerase chain reaction (PCR). Methods. Blood samples were collected from 50 dogs and 50 cats in Botucatu-SP, Brazil. For hemoculture, the samples were inoculated in LIT medium, and readings were performed for four months. Upon completion of such period, all the hemocultures were processed for parasitic DNA extraction. The PCR reactions were performed by using primers TCZ1/TCZ2. Results. Ten dogs and ten cats (20%) were positive to PCR, and four dogs and three cats (7%) were positive to hemoculture. Only in a one cat sample (1%) there was confirmation of positive hemoculture by PCR for T. cruzi. Conclusions. Results showed that PCR was a suitable tool for the confirmation of the parasite detection in hemoculture samples, and that dogs and cats from Botucatu, SP, Brazil, are maintaining the role of household reservoirs of T. cruzi, which reinforces the need for constant epidemiologic surveillance for this zoonosis.