Table of Contents
ISRN Analytical Chemistry
Volume 2012, Article ID 423572, 8 pages
Research Article

Liquid Chromatography Study on Atenolol- 𝜷 -Cyclodextrin Inclusion Complex

1Department of Chemistry, KSKV Kachchh University, Gujarat, Bhuj 370001, India
2Department of Chemistry, School of Science, Gujarat University, Gujarat, Ahmedabad 380009, India

Received 25 October 2011; Accepted 5 December 2011

Academic Editors: D. Kara, B. Rittich, and G. Vecchio

Copyright © 2012 Shailesh M. Buha et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Inclusion complex formation of atenolol with 𝛽 -cyclodextrin ( 𝛽 -CD) has been investigated by HPLC on different stationary phases, by varying pH and concentration of 𝛽 -CD added as an additive in the mobile phase over a wide range of column temperature. Stationary phases of different polarity and hydrophobicity were evaluated to find the best conditions for complex formation. The optimum conditions for inclusion complexation were achieved on YMC ODS-AQ C18 ( 1 5 0 Γ— 4 . 6  mm, 5 μ) analytical column. The apparent formation constant ( 𝐾 ) of the complex as evaluated by liquid chromatography using retention factors ( π‘˜ ) was 1 7 9 . 4 7 Β± 2 . 5  M−1 at 25°C. The stoichiometry of the complex was 1 : 1 as is evident from the straight line plot of 1/ π‘˜ versus 𝛽 -CD concentration. The formation of inclusion complex was essentially enthalpy (−42.12 kJ/mol) driven and the binding forces included hydrophobic, van der Waals-London dispersion interactions. The enthalpy-entropy compensation criterion was used to prove the inclusion phenomena.