Liquid Chromatography Study on Atenolol--Cyclodextrin Inclusion Complex
Table 2
Effect of pH on different columns and apparent formation constants of atenolol. Mobile phase: 50 mM NaH2PO4, pH—6.8: methanol (92 : 8, v/v). Wavelength: 226 nm. Flow rate: 1.0 mL/min. Temperature: 25°C.
Analytical column
Retention time, min
Retention factor,
Apparent formation constant, (M−1)
0 mM β-CD
10 mM β-CD
0 mM β-CD
10 mM β-CD
pH 3.0
aKromasil, C4
8.4
6.3
3.33
2.23
49.33
bKromasil, NH2
3.2
3.1
0.14
0.09
55.56
cYMC-CN
5.0
4.8
0.56
0.50
12.00
dYMC ODS-AQ C18
12.8
6.5
6.07
2.59
134.36
pH 4.0
aKromasil, C4
9.2
6.7
3.72
2.44
52.46
bKromasil, NH2
3.7
3.3
0.32
0.18
77.78
cYMC-CN
6.1
5.6
0.91
0.75
21.33
dYMC ODS-AQ C18
14.6
7.1
7.06
2.92
141.78
pH 5.0
aKromasil, C4
11.6
7.5
5.18
2.85
73.68
bKromasil, NH2
4.9
3.9
0.75
0.39
92.31
cYMC-CN
8.4
6.9
1.63
1.16
40.52
dYMC ODS-AQ C18
17.8
7.8
8.83
3.31
166.77
pH 6.8
aKromasil, C4
13.5
8.4
5.92
3.32
78.31
bKromasil, NH2
5.8
4.3
1.07
0.52
98.15
cYMC-CN
10.6
8.0
2.31
1.51
52.98
dYMC ODS-AQ C18
22.8
9.3
11.62
4.16
179.47
Dead time of the columns: a1.94 min, b2.80 min, c3.20 min, d1.81 min. All measurements were done in triplicate.