Liquid Chromatography Study on Atenolol-𝜷-Cyclodextrin Inclusion Complex

Table 4

Apparent formation constant (𝐾) of atenolol and thermodynamic parameters at different temperatures in presence of β-CD. Column: YMC ODS-AQ C18 (150 × 4.6 mm, 5 μ). Mobile phase: 10 mM β-CD in 50 mM NaH₂PO₄, pH—6.8: methanol (92 : 8, v/v). Wavelength: 226 nm. Flow rate: 1.0 mL/min.

Temperature, K	Apparent formation constant, 𝐾 (M^−1)	Δ𝐺^0 (kJ/mol)	Δ𝐻^0 (kJ/mol)	Δ𝑆^0 (kJ/K/mol)	𝑇Δ𝑆^0 (kJ/mol)
288	324.44	−13.85	—	—	—
293	239.85	−13.35	−42.40	−0.0933	−29.05
298	179.47	−12.86	−42.12	−0.0982	−29.26
303	135.64	−12.37	−42.05	−0.0979	−29.68
308	103.54	−11.88	−41.92	−0.0975	−30.04
313	79.84	−11.40	−41.68	−0.0967	−30.28

All measurements were done in triplicate.