Liquid Chromatography Study on Atenolol-𝜷-Cyclodextrin Inclusion Complex

Table 5

Retention factors of atenolol and thermodynamic parameters at different temperatures in absence of β-CD. Column: YMC ODS-AQ C18 (150 × 4.6 mm, 5 μ). Mobile phase: 50 mM NaH₂PO₄, pH—6.8: methanol (92 : 8, v/v). Wavelength: 226 nm. Flow rate: 1.0 mL/min.

Temperature, K	Retention factor, 𝑘	Δ𝐺^0 (kJ/mol)	Δ𝐻^0 (kJ/mol)	Δ𝑆^0 (kJ/K/mol)	𝑇Δ𝑆^0 (kJ/mol)
288	14.62	−6.42	—	—	—
293	13.00	−6.25	−16.48	−0.0349	−10.23
298	11.62	−6.08	−16.35	−0.0350	−10.27
303	10.43	−5.91	−16.23	−0.0341	−10.32
308	9.40	−5.74	−16.14	−0.0337	−10.40
313	8.51	−5.57	−16.04	−0.0334	−10.47

All measurements were done in triplicate.