Capsaicin, a TRPV1 Ligand, Suppresses Bone Resorption by Inhibiting the Prostaglandin E Production of Osteoblasts, and Attenuates the Inflammatory Bone Loss Induced by Lipopolysaccharide
Figure 2
Effects of capsaicin on the production of PGE2 and the expression of COXs and mPGES-1 mRNAs in mouse primary osteoblasts. (a) Mouse osteoblasts were cultured with LPS (1 ng/mL) for 3 h with or without capsaicin (30 μM). Total RNA was extracted, and the expression of COX-1, COX-2, and mPGES-1 mRNAs was detected using RT-PCR. The relative intensity of mRNA expression was calculated and noted in the upper of respective signal. (b) Osteoblasts were treated with 30 μM capsaicin in the presence of LPS (1 ng/mL) for 24 h. The levels of PGE2 were measured using the conditioned medium. Data are expressed as the mean ± SEM of 3 independent wells. A significant difference between the two groups is indicated, * versus control; ## versus LPS.
