Evaluation of Viability and Proliferation Profiles on Macrophages Treated with Silica Nanoparticles In Vitro via Plate-Based, Flow Cytometry, and Coulter Counter Assays
Figure 4
Proliferation assays show contradictory results among methods. RAW 264.7 cells were plated in DMEM-supplemented media, and cells were processed differently dependent on the assay (as described in Section 2). (a) There is an increased proliferation in cells treated with g/L SiO2 NP; punctuated line shows control value (100%). (b) There is a decrease in cell number in cells exposed to SiO2 NP (0.005 g/L and 0.01 g/L) and LPS at 24 h. (c) CFSE staining shows a nonsignificant delay in cell proliferation. Punctuated line shows the peak in CFSE intensity in control sample. High CFSE intensity (as shown in CFSE-stained cells on day 1 and CFSE-stained cells cultured with no FBS) indicates reduced cell proliferation. Experiment was performed 3 times, and one representative histogram is shown. (a) and (b) show average with SEM ( in (a) and samples in (b)) from different experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey’s posttest, and * is considered significant when comparing SiO2 NPs-treated cells to control.
