Culture Condition Improvement for Phytase Production in Solid State Fermentation by Aspergillus ficuum Using Statistical Method

Table 1

Experimental variables at different levels used for the production of phytase by Aspergillus ficuum using Plackett-Burman design.

Variables	Unit	Low level	High level
		(−)	(+)
Moisture (A)	%	50	70
Temperature (B)	°C	25	35
Particle size (C)	Mm	0.2–0.6	1.0–1.4
Time (D)	Hour	48	144
MgSO4 (E)	g/g dry substrate %	0	0.3
KH2PO4 (F)	g/g dry substrate %	0	0.01
(NH4)2SO4 (G)	g/g dry substrate %	2	6
Glucose (H)	g/g dry substrate %	5	15
Tween-80 (I)	g/g dry substrate %	0	0.5
NaCl (J)	g/g dry substrate %	0	0.3
Inoculum size (K)	mL	0.5	2.5