Culture Condition Improvement for Phytase Production in Solid State Fermentation by Aspergillus ficuum Using Statistical Method

Table 2

Plackett-Burman experimental design for screening of significant process variables affecting phytase production.

Run order	Variables											Phytase activity (U/gds)
	A	B	C	D	E	F	G	H	I	J	K	Replication 1	Replication 2
1	1	1	−1	1	1	−1	1	−1	−1	−1	1	15.0	13.3
2	−1	−1	1	1	1	−1	1	1	−1	1	−1	13.6	11.5
3	−1	1	1	−1	1	−1	−1	−1	1	1	1	0.2	0.0
4	−1	−1	−1	−1	−1	−1	−1	−1	−1	−1	−1	1.2	1.0
5	1	−1	1	1	−1	1	−1	−1	−1	1	1	6.0	3.8
6	1	1	−1	1	−1	−1	−1	1	1	1	−1	9.1	9.5
7	1	−1	−1	−1	1	1	1	−1	1	1	−1	3.3	2.7
8	1	−1	1	−1	−1	−1	1	1	1	−1	1	1.2	1.1
9	−1	−1	−1	1	1	1	−1	1	1	−1	1	8.9	9.4
10	−1	1	1	1	−1	1	1	−1	1	−1	−1	0.9	1.3
11	1	1	1	−1	1	1	−1	1	−1	−1	−1	5.8	5.3
12	−1	1	−1	−1	−1	1	1	1	−1	−1	−1	0.0	0.0