Culture Condition Improvement for Phytase Production in Solid State Fermentation by Aspergillus ficuum Using Statistical Method

Table 4

Exprimental design and the results of central composite design for phytase production.

Run	𝑋1	𝑋2	𝑋3	𝑋4	Phytase activity (U/gds)
					Observed	Predicted
1	−1	−1	−1	−1	22.4	21.95
2	−1	−1	−1	1	20.6	21.39
3	−1	−1	1	−1	19.4	19.52
4	−1	−1	1	1	21.6	22.00
5	−1	1	−1	−1	21.5	21.35
6	−1	1	−1	1	20.0	20.79
7	−1	1	1	−1	18.7	18.92
8	−1	1	1	1	21.2	21.40
9	1	−1	−1	−1	18.4	18.52
10	1	−1	−1	1	16.9	17.20
11	1	−1	1	−1	17.0	16.74
12	1	−1	1	1	18.0	18.47
13	1	1	−1	−1	18.2	18.32
14	1	1	−1	1	16.8	17.00
15	1	1	1	−1	17.0	16.54
16	1	1	1	1	17.3	18.27
17	−2	0	0	0	20.3	19.76
18	2	0	0	0	13.5	13.19
19	0	−2	0	0	23.3	22.98
20	0	2	0	0	22.7	22.18
21	0	0	−2	0	22.0	21.56
22	0	0	2	0	20.8	20.39
23	0	0	0	−2	15.7	16.49
24	0	0	0	2	19.3	17.66
25	0	0	0	0	24.4	23.87
26	0	0	0	0	24.0	23.87
27	0	0	0	0	23.2	23.87
28	0	0	0	0	23.9	23.87