Implication of NADPH Oxidases in the Early Inflammation Process Generated by Cystic Fibrosis Cells
Figure 1
Oxidative stress in IB3-1 defective CFTR cells as compared with S9 control cells. Cells were treated or not by DPI (2.5 M) before incubation with the isotonic, hypotonic, or hypertonic media (a) ROS production was measured using a luminol-amplified chemiluminescence method as described in Section 2. Results were obtained in cpm/106 cells/2 h and were expressed as the percentage of chemiluminescence intensity obtained for each cell type in hypotonic and hypertonic conditions as compared to isotonic conditions. Chemiluminescence of IB3-1 and S9 cells incubated in isotonic conditions was (11.3 ± 1.5) 106 and (11.4 ± 1.2) 106, respectively. (b) GSH content of IB3-1 defective CFTR cells and S9 control cells was measured as described in Section 2. NEM, which blocks sulfhydryl groups, was used as control. Results were obtained in arbitrary fluorescence units (AFUs) and were expressed as the percentage of AFUs obtained for each cell type in hypotonic and hypertonic conditions as compared with that obtained in isotonic conditions. AFUs of IB3-1 and S9 cells incubated in isotonic conditions were 34855 ± 1320 and 38870 ± 1637, respectively. Results are mean ± SEM (). significantly different from S9 in the same conditions (); *significantly different from IB3-1 or S9 in isotonic medium, respectively (); #significantly different from nontreated with DPI in the same medium ( ).
