Implication of NADPH Oxidases in the Early Inflammation Process Generated by Cystic Fibrosis Cells
Figure 4
DUOX1/2 and NOX2 proteins expression in IB3-1 defective CFTR cells and in S9 control cells. DUOX1/2 were detected with an apparent molecular weight of 175 kDa by SDS PAGE and immunoblotting using a rabbit polyclonal antibody directed against human DUOX1/2 as described in Section 2. The human lung mucoepidermoid carcinoma derived cell line NCI-H292 was used as control for DUOX1 and DUOX2 positive cells. Neutrophils isolated from human blood were used as controls for NOX2 (gp91phox), p22phox, p47phox, and p67phox expression, which were detected by SDS-PAGE and immunoblotting using antibodies described in Section 2. actin was used as a loading control. (a) Basal expression of DUOX1/2 in IB3-1 and S9 cells cultured in isotonic medium. (b) Basal expression of gp91phox (NOX2), p22phox, p47phox, and p67phox in IB3-1 and S9 cells. Representative of three experiments. Dotted lines indicate that intervening lanes have been spliced out.
