Implication of NADPH Oxidases in the Early Inflammation Process Generated by Cystic Fibrosis Cells
Figure 5
Effect of calcium ionophore A23187 in presence or absence of diphenyleneiodonium (DPI) on ROS production, induction of apoptosis, and IL-6 and IL-8 production by S9 and IB3-1 cells. S9 and IB3-1 cells were incubated in isotonic culture medium alone (control) or in medium with 5 M calcium ionophore in presence (A23187 + DPI) or absence (A23187) of 2.5 M DPI. (a) Luminol-amplified chemiluminescence. Results were expressed in relative ratio to control S9 or IB3-1 cells. (b) Apoptosis was measured using Yopro-1 as described in Section 2. Results were expressed in relative ratio to control S9 or IB3-1 cells. (c) IL-6 and (d) IL-8 production by S9 and IB3-1 cells as described in Section 2. Results were expressed as pg/mL. Results are expressed as mean ± SEM, . significantly different from S9 in the same conditions (); *significantly different from IB3-1 or S9 in control isotonic medium, respectively (); #significantly different from A23187 alone ().
