Table of Contents
ISRN Cardiology
Volume 2012 (2012), Article ID 483407, 5 pages
http://dx.doi.org/10.5402/2012/483407
Research Article

Distribution of Cardiac Stem Cells in the Human Heart

1Department of Cardiac Surgery, Kerckhoff Klinik, Bad Nauheim, Benekestr. 2-8, 61231 Bad Nauheim,, Germany
2Department of Cardiology, Heart Center Leipzig, University of Leipzig, Struempellstrasse 39, 04289 Leipzig, Germany
3Department of Cardiac Surgery, Heart Center Leipzig, University of Leipzig, Struempellstrasse 39, 04289 Leipzig, Germany

Received 30 September 2011; Accepted 13 November 2011

Academic Editor: F. Quaini

Copyright © 2012 Mani Arsalan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. The existence of human cardiac stem cells (hCSC) and their regenerative capacity are not fully defined. The aim of this study was to identify and analyse the distribution of hCSCs by flow cytometry (FCM). Methods. Tissue samples from the left ventricle (LV) and the appendages of the right atrium (RA) and left atrium (LA) were taken during cardiac surgery. Mononuclear cells (MNCs) were isolated, labelled for the stem-cell-marker c-kit and hematopoietic-lineage markers and analysed by FCM. Results. HCSCs could be isolated from the RA, LA, and LV without significant quantitative difference between both atria (A) (RA 4.80 ± 1.76% versus LA 4.99 ± 1.69% of isolated MNCs, 𝑃 = 0 . 9 2 2 ). The number of hCSCs was significantly higher in both atria compared to the left ventricle (A 4.90 ± 1.29% versus LV 0.62 ± 0.14% of isolated MNCs, 𝑃 = 0 . 0 3 5 ). Conclusion. The atria contain a higher concentration of hCSC than the left ventricle. HCSCs located in the atria could serve as an endogenous source for heart regeneration.