Table of Contents
ISRN Cell Biology
Volume 2012 (2012), Article ID 513546, 9 pages
http://dx.doi.org/10.5402/2012/513546
Research Article

An Approach to Visualize the Deformation of the Intermediate Filament Cytoskeleton in Response to Locally Applied Forces

Department of Physics and Department of Biology, University of Ottawa, MacDonald Hall,150 Louis Pasteur, Ottawa, ON, Canada K1N 6N5

Received 28 August 2011; Accepted 25 September 2011

Academic Editors: P. Lavia and C. C. Uphoff

Copyright © 2012 Jiashan Wang and Andrew E. Pelling. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. Huang, R. D. Kamm, and R. T. Lee, “Cell mechanics and mechanotransduction: pathways, probes, and physiology,” American Journal of Physiology, vol. 287, no. 1, pp. C1–C11, 2004. View at Publisher · View at Google Scholar · View at Scopus
  2. A. J. Engler, M. A. Griffin, S. Sen, C. G. Bönnemann, H. L. Sweeney, and D. E. Discher, “Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments,” The Journal of Cell Biology, vol. 166, no. 6, pp. 877–887, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. A. J. Engler, S. Sen, H. L. Sweeney, and D. E. Discher, “Matrix elasticity directs stem cell lineage specification,” Cell, vol. 126, no. 4, pp. 677–689, 2006. View at Publisher · View at Google Scholar · View at Scopus
  4. I. Andricioaei, A. Goel, D. Herschbach, and M. Karplus, “Dependence of DNA polymerase replication rate on external forces: a model based on molecular dynamics simulations,” Biophysical Journal, vol. 87, no. 3, pp. 1478–1497, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. N. Wang, J. P. Butler, and D. E. Ingber, “Mechanotransduction across the cell surface and through the cytoskeleton,” Science, vol. 260, no. 5111, pp. 1124–1127, 1993. View at Google Scholar · View at Scopus
  6. J. Bertaud, Z. Qin, and M. J. Buehler, “Intermediate filament-deficient cells are mechanically softer at large deformation: a multi-scale simulation study,” Acta Biomaterialia, vol. 6, no. 7, pp. 2457–2466, 2010. View at Publisher · View at Google Scholar · View at Scopus
  7. Z. Qin, L. Kreplak, and M. J. Buehler, “Hierarchical structure controls nanomechanical properties of vimentin intermediate filaments,” PLoS One, vol. 4, no. 10, Article ID e7294, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. Z. Qin, L. Kreplak, and M. J. Buehler, “Nanomechanical properties of vimentin intermediate filament dimers,” Nanotechnology, vol. 20, no. 42, Article ID 425101, 2009. View at Publisher · View at Google Scholar · View at Scopus
  9. G. W. Brodland and R. Gordon, “Intermediate filaments may prevent buckling of compressively loaded microtubules,” Journal of Biomechanical Engineering, vol. 112, no. 3, pp. 319–321, 1990. View at Google Scholar · View at Scopus
  10. K. J. Green, M. Böhringer, T. Gocken, and J. C. R. Jones, “Intermediate filament associated proteins,” Advances in Protein Chemistry, vol. 70, pp. 143–202, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. S. D. Georgatos and G. Blobel, “Lamin B constitutes an intermediate filament attachment site at the nuclear envelope,” The Journal of Cell Biology, vol. 105, no. 1, pp. 117–125, 1987. View at Google Scholar · View at Scopus
  12. A. J. Maniotis, C. S. Chen, and D. E. Ingber, “Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure,” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 3, pp. 849–854, 1997. View at Publisher · View at Google Scholar · View at Scopus
  13. P. A. Janmey, U. Euteneuer, P. Traub, and M. Schliwa, “Viscoelastic properties of vimentin compared with other filamentous biopolymer networks,” The Journal of Cell Biology, vol. 113, no. 1, pp. 155–160, 1991. View at Google Scholar · View at Scopus
  14. L. Kreplak and D. Fudge, “Biomechanical properties of intermediate filaments: from tissues to single filaments and back,” BioEssays, vol. 29, no. 1, pp. 26–35, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. T. Ackbarow, D. Sen, C. Thaulow, and M. J. Buehler, “Alpha-helical protein networks are self-protective and flaw-tolerant,” PLoS One, vol. 4, no. 6, Article ID e6015, 2009. View at Publisher · View at Google Scholar · View at Scopus
  16. G. Binnig, C. F. Quate, and C. Gerber, “Atomic force microscope,” Physical Review Letters, vol. 56, no. 9, pp. 930–933, 1986. View at Publisher · View at Google Scholar · View at Scopus
  17. H. Haga, S. Sasaki, K. Kawabata, E. Ito, T. Ushiki, and T. Sambongi, “Elasticity mapping of living fibroblasts by AFM and immunofluorescence observation of the cytoskeleton,” Ultramicroscopy, vol. 82, no. 1–4, pp. 253–258, 2000. View at Publisher · View at Google Scholar · View at Scopus
  18. M. Horton, G. Charras, C. Ballestrem, and P. Lehenkari, “Integration of atomic force and confocal microscopy,” Single Molecules, vol. 1, no. 2, pp. 135–137, 2000. View at Google Scholar · View at Scopus
  19. P. P. Lehenkari, G. T. Charras, S. A. Nesbitt, and M. A. Horton, “New technologies in scanning probe microscopy for studying molecular interactions in cells,” Expert Reviews in Molecular Medicine, vol. 2, no. 2, pp. 1–19, 2000. View at Google Scholar
  20. P. Kunda, A. E. Pelling, T. Liu, and B. Baum, “Moesin controls cortical rigidity, cell rounding, and spindle morphogenesis during mitosis,” Current Biology, vol. 18, no. 2, pp. 91–101, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. A. E. Pelling, F. S. Veraitch, C. P. K. Chu, C. Mason, and M. A. Horton, “Mechanical dynamics of single cells during early apoptosis,” Cell Motility and the Cytoskeleton, vol. 66, no. 7, pp. 409–422, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. P. P. Lehenkari, G. T. Charras, A. Nykänen, and M. A. Horton, “Adapting atomic force microscopy for cell biology,” Ultramicroscopy, vol. 82, no. 1–4, pp. 289–295, 2000. View at Publisher · View at Google Scholar · View at Scopus
  23. A. Kölsch, R. Windoffer, T. Würflinger, T. Aach, and R. E. Leube, “The keratin-filament cycle of assembly and disassembly,” Journal of Cell Science, vol. 123, no. 13, pp. 2266–2272, 2010. View at Publisher · View at Google Scholar · View at Scopus
  24. R. Matzke, K. Jacobson, and M. Radmacher, “Direct, high-resolution measurement of furrow stiffening during division of adherent cells,” Nature Cell Biology, vol. 3, no. 6, pp. 607–610, 2001. View at Publisher · View at Google Scholar · View at Scopus
  25. H. Ngu, Y. Feng, L. Lu, S. J. Oswald, G. D. Longmore, and F. C. P. Yin, “Effect of focal adhesion proteins on endothelial cell adhesion, motility and orientation response to cyclic strain,” Annals of Biomedical Engineering, vol. 38, no. 1, pp. 208–222, 2010. View at Publisher · View at Google Scholar · View at Scopus
  26. A. P. Zhu and N. Fang, “Adhesion dynamics, morphology, and organization of 3T3 fibroblast on chitosan and its derivative: the effect of O-carboxymethylation,” Biomacromolecules, vol. 6, no. 5, pp. 2607–2614, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. R. Kalluri and M. Zeisberg, “Fibroblasts in cancer,” Nature Reviews Cancer, vol. 6, no. 5, pp. 392–401, 2006. View at Publisher · View at Google Scholar · View at Scopus
  28. M. Yoon, R. D. Moir, V. Prahlad, and R. D. Goldman, “Motile properties of vimentin intermediate filament networks in living cells,” The Journal of Cell Biology, vol. 143, no. 1, pp. 147–157, 1998. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Plodinec, M. Loparic, R. Suetterlin, H. Herrmann, U. Aebi, and C.-A. Schoenenberger, “The nanomechanical properties of rat fibroblasts are modulated by interfering with the vimentin intermediate filament system,” Journal of Structural Biology, vol. 174, no. 3, pp. 476–484, 2011. View at Publisher · View at Google Scholar
  30. C. L. Ho, J. L. Martys, A. Mikhailov, G. G. Gundersen, and R. K. H. Liem, “Novel features of intermediate filament dynamics revealed by green fluorescent protein chimeras,” Journal of Cell Science, vol. 111, part 13, pp. 1767–1778, 1998. View at Google Scholar · View at Scopus