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ISRN Pharmacology
Volume 2012 (2012), Article ID 515634, 4 pages
Research Article

In Vitro  α-Amylase Inhibitory Activity of the Leaves of Thespesia populnea

Department of Biochemistry, School of Life Sciences, Vels University, Chennai 600 117, India

Received 26 November 2011; Accepted 10 January 2012

Academic Editors: S. Ceruti and K. Cimanga

Copyright © 2012 R. Sangeetha and N. Vedasree. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Postprandial hyperglycemia is a prime characteristic of diabetes mellitus and has been a focus in the therapy for diabetes. One of the therapeutic approaches which involve decreasing hyperglycemia aims at inhibiting the enzyme α-amylase. The leaves of T. populnea were studied for the presence of amylase inhibitors. The fractions obtained by successive fractionation using solvents of varying polarity were studied for the presence of primary and secondary metabolites. The total phenolic content of the different fractions was determined by HPLC and was correlated with their amylase inhibitory potential. Similarly, the protein content of the extracts was also estimated to understand the nature of the inhibitor present. This study shows that the leaves of T. populnea were effective in inhibiting α-amylase, thereby proving to be potential hyperglycemic agents.