Figure 1: (a) 25 mM D-glucose (HG) caused decreased miRNA-320 expression in the endothelial cells compared to 5 mM D-glucose (LG) by real-time RT-PCR. (b) Similarly, diabetes caused reduced miR-320 expression in the kidney. (miRNA expressed as a ratio of U6 snRNA (U6). 𝑃 < 0 . 0 5 compared to LG or control; 𝑛 = 6 /treatment; analyses were performed using mirVana miRNA isolation kit and TaqMan microRNA assay system).