Stability Indicating Method for Simultaneous RP HPLC Determination of Camylofin Dihydrochloride and Nimesulide in Pharmaceutical Preparations

Table 1

Optimization of LC method.

Mobile Phase used	Column used	Flow rate	Observation	Result
DI water : acetonitrile (500 : 500)	Waters symmetry C18, 250 × 4.6, 5 μ	1.0 mL min^−1	No peaks observed	Method (rejected)
DI water : acetonitrile (500 : 500)	Varian Chromosphere 5 C18, 250 × 4.6, 5 μ	1.5 mL min^−1	Long run time and improper peak shape	Method (rejected)
DI water : methanol (500 : 500)	Varian Chromosphere 5 C18, 250 × 4.6, 5 μ	1.5 mL min^−1	Poor resolution and bad peak shape	Method (rejected)
Buffer solution pH5.0 : methanol (500 : 500)	Varian Chromosphere 5 C18, 250 × 4.6, 5 μ	1.0 mL min^−1	Low resolution between first two peaks (<2.0)	Method (rejected)
Buffer solution pH5.0 : methanol (500 : 500)	Varian Chromosphere 5 C18, 250 × 4.6, 5 μ	1.0 mL min^−1	Good resolution and great peak shape	Method (accepted)