International Scholarly Research Notices

Research Article

Molecular Characterisation of Bacterial Community Structure along the Intestinal Tract of Zebrafish (Danio rerio): A Pilot Study

Table 1

Comparison of bacterial phyla identified in the study by Roeselers et al. [19] and this study.
PhylumSanger sequencing clone librariesPyrosequencing librariesThis studya
Proteobacteria 7 9 . 4 1 % ± 1 6 . 2 9 % b 6 0 . 5 1 % ± 3 5 . 3 9 % c 5 9 . 2 0 % ± 1 7 . 0 3 % d
Firmicutes 1 . 0 1 % ± 1 . 2 2 % 6 . 3 6 % ± 7 . 5 4 % 2 3 . 8 1 % ± 1 0 . 7 9 %
Bacteroidetes 0 . 0 5 % ± 0 . 1 2 % 0 . 8 8 % ± 0 . 8 0 % Not found
Verrucomicrobia 0 . 0 5 % ± 0 . 1 4 % Not found 0 . 1 9 % ± 0 . 3 4 %
Actinobacteria 1 . 0 9 % ± 2 . 3 9 % 2 . 8 5 % ± 4 . 6 8 % 4 . 4 0 % ± 1 . 2 4 %
TM7 0 . 0 2 % ± 0 . 0 5 % Not found 0 . 5 2 % ± 0 . 4 9 %
Fusobacteria 1 3 . 6 4 % ± 1 4 . 3 5 % 1 3 . 3 1 % ± 2 0 . 8 2 % 8 . 0 1 % ± 4 . 4 1 %
Planctomycetes 1 . 5 2 % ± 2 . 5 3 % 0 . 0 6 % ± 0 . 1 0 % 1 . 0 5 % ± 0 . 5 1 %
Nitrospira 0 . 0 3 % ± 0 . 0 9 % Not found 0 . 3 9 % ± 0 . 6 8 %
Deinococcus-ThermusNot found 0 . 3 3 % ± 0 . 5 5 % Not found
TenericutesNot foundNot found 0 . 1 9 % ± 0 . 3 4 %
Acidobacteria 0 . 0 4 % ± 0 . 0 9 % Not found 0 . 1 9 % ± 0 . 3 4 %
Cyanobacteria 2 . 7 1 % ± 7 . 1 6 % 1 5 . 1 4 % ± 2 5 . 5 3 % Not found
Unclassified bacteria 0 . 3 9 % ± 0 . 5 4 % 0 . 5 4 % ± 0 . 7 6 % 2 . 0 4 % ± 1 . 7 8 %
aIn this study, % of each phylum in the clone libraries (IB, MI, and PI) was derived by pooling clones from different compartments from each fish. b M e a n ± S D of clone libraries ( 𝑛 = 7 ). The seven libraries include D.rerio.UNC.1, D.rerio.India.1, D.rerio.ZIRC.1, D.rerio.UO.1, D.rerio.WU.1, D.rerio.WU.2, and D.rerio.UW.1. c M e a n ± S D of libraries ( 𝑛 = 3 ). The three libraries include D.rerio.India.1, D.rerio.UNC.1, and D.rerio.UW.1. d M e a n ± S D of pooled clone libraries ( 𝑛 = 3 ).